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Comprehensive Protocol Collection
Comprehensive collection of molecular biology and Nematode-related protocols, including many standard biological assays & stainings.
http://www.dartmouth.edu/artsci/bio/ambros/protocols.html
BBHelp
Collection of molecular biology techniques derived from help files of Dr. Harold Zappe at the Department of Medical Microbiology, University of Cape Town, South Africa.
http://www.uct.ac.za/depts/mmi/bbhelp/intro.html
Basic Principles of Gene Cloning, Sequencing, & Analysis
Description of methods behind gene cloning, genetic engineering, DNA recombination and isolation, including discussion of restriction enzymes and ligase.
http://www.geocities.com:80/SiliconValley/5504/biochem.html
Tronolab Website
Collection of protocols, maps and sequences regarding manipulation of lentiviral vectors, siRNA and transgenic animals. Applications in HIV, stem cell and gene therapy research at University of Geneva.
http://www.tronolab.com/
Clontechniques
Quarterly newsletter, with search to access new product information and applications research updates and earlier articles from of Clontech, now in the BD BioSciences Group, at Palo Alto, CA.
http://www.bdbiosciences.com/clontech/archive/index.shtml
ECR Browser
Multiple alignment and dynamic evolutionary conservation profile of several vertebrate genomes including human, mouse, rat, chicken, frog, and three fishes, developed at LLNL, Livermore, California.
http://ecrbrowser.dcode.org/
iProtocol for Bioscience
Key protocols used in biochemistry, bioengineering, cytobiology, genetics and molecular biology at MIT, Cambridge, MA.
http://iprotocol.mit.edu
Comet Assay Interest Group
Forum for exchange of views on the single cell gel electrophoresis system, capable of detecting single- and double-strand breaks, oxidative DNA base damage, and DNA, protein and drug crosslinking. Includes related links.
http://cometassay.com
SciWeb Protocols and Reagents
Directory of molecular biology protocol links.
http://sciweb.com/features/protocols.cfm
Molecular Biology Protocols
Resources such as polymerase chain reaction, dedicated to method development. Part of internet directory maintained by Horizon Scientific Press, Wymondham, UK.
http://highveld.com/protocols.html
MolBio-Spot
Richard Man-kit Yu's site where you can acquire various molecular biology techniques.
http://www.geocities.com/richyuhk/
Assay of Protein Concentration
Different methods of determining protein concentration, as a resource for researchers and students in biochemistry and molecular biology laboratories from University of Nebraska, Lincoln, NE.
http://www-class.unl.edu/biochem/protein_assay/
Molecular Probes, Inc.
Handbook of fluorescence applications, bibliography, data tables and calculators for molecular biology, information on courses and workshops from supplier in Eugene, OR and Leiden, Netherlands.
http://www.probes.com/resources/
Practical Molecular Biology
Methods, information, programs and BioMail for molecular biologists, as joint venture between Germany and Russia.
http://molbiol.ru/eng/
Molecular Cloning Manual
Subscription only source of laboratory techniques in DNA and protein science, to complement the hard copy, giving scientists the benefit of both media from CSHL, NY.
http://www.molecularcloning.com/
Molbiol.net
Resource for molecular biologists on methods, with feedback for user-directed evolution and links to commercial suppliers from Jonathan D Rees, Oxford, UK.
http://www.molbiol.net/
Comparative Genomic Hybridization
CGH is a molecular cytogenetic method of screening a tumor for genetic changes. CGH protocols, images, publications, events and links maintained by the University Hospital Charité, Humboldt-University of Berlin, Germany.
http://amba.charite.de/cgh/
Laboratory Dialysis Answers
Discussion board for laboratory dialysis tips and techniques, moderated by Spectrum Laboratories, Inc.
http://www.labdialysischat.com/
Biomedpage
Collection of laboratory protocols, applications and links to publications in biomedicine and molecular biology from author in Germany.
http://www.biomedpage.de/indexe.html
Reto's Buffers and Media Book
Alphabetical list for buffer recipes, including constituents for cultures, from personal page in Switzerland.
http://www.ikp.unibe.ch/~rportman/buffersmedia.html
Owczarzy, Richard
Online calculations of nucleic acid extinction coefficients, melting temperatures, and links to biophysics databases. Includes thesis, publications and contacts for commercial scientist in Coralville, Iowa.
http://www.owczarzy.net/
Biopolymer Calculator
Calculates molecular weight, composition and concentration of nucleic acids and proteins, based on provided sequence, dilution and absorbance measurement. Also melting temperatures for nucleic acids. Maintained at Schepartz Laboratory of Chemical Biology, Yale, New Haven, CT.
http://paris.chem.yale.edu/extinct.html
Molecular Biology Protocols
Protocol collection, with interactive forum for over ten topics, covering DNA, PCR, RNA and proteins techniques and links to online journals and bioinformatics resources, from the Northwest Fisheries Science Center Microbial Pathogenesis Lab, Seattle, WA.
http://micro.nwfsc.noaa.gov/protocols/
biowww.net: Molecular Biology
Comprehensive trouble shooting archive, with protocol and newsgroup links, including cell biology, immunology and genetics.
http://www.biowww.net/index.php/article/archive/9/
BioProtocol
Internet project initiated in early 1992 as BRNI, bio.com includes PCR, hybridization and microarray protocols.
http://bioprotocol.bio.com/protocolstools/
Home Page of Andy Vierstraete
The use of DNA techniques in phylogenesis. Explanations, protocols and animations from Andy Vierstraete at the University of Ghent, Belgium.
http://allserv.ugent.be/~avierstr/
Surgery Research
Collection of DNA microarray technology protocols, primarily for resident students at Stanford.
http://surgeryresearch.com/
Beginning Laboratory Manual
Teaching manual to support the applied biotechnology programs at the University of Maryland, Baltimore.
http://www.research.umbc.edu/~jwolf/method1.html
Recombinant DNA
Preparing probe, cloning into plasmids, complementarity and hybridization, gel electrophoresis, transfer to solid support and blotting variants, blocking, target detection PCR problems from chapter in MIT hypertext handbook.
http://web.mit.edu/esgbio/www/rdna/rdnadir.html
Hirsh Lab Gel Documentation System
Description of an agarose gel set-up. Includes instructions for camera operation with a script, with computer side image analysis from the University of Virginia, Charlottesville.
http://www.virginia.edu/biology/Fac/hirsh_gel/gel/
Bioscientific Research
Personal page with hybridization, extraction and plant expression vectors and library suggestions.
http://www.biosc.org/interest.htm
Protocols
Microsatellites and others techniques from the Savana River Ecology Laboratory, University of Georgia.
http://www.uga.edu/srel/DNA_Lab/protocols.htm
SNP Scoring
Overview of genotyping, detection and quantifying single nucleotide polymorphism, including the application of template directed dye terminator incorporation assay. Offers database and links to other resources from PerkinElmer Life Sciences, Boston, MA.
http://las.perkinelmer.com/catalog/Category.aspx?CategoryName=SNP+Scoring
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